Tissue regeneration: Researchers create gel that regrows tooth enamel

…and eliminates pain associated with tooth decay

Our article “How to Heal Cavities Naturally” has triggered an ongoing discussion ever since it was posted.

However, science has developed a new technique that holds the promise of tooth regrowth.

Researchers say tooth gel will regrow enamel and cover painful blemishes.

Dual discoveries in tissue regeneration at the University of Southern California propose a promising method to regrow nonliving hard tissue, lessening or even eliminating pain associated with tooth decay, which the National Institutes of Health calls the most prevalent chronic disease.

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Nearly grown enamel

Janet Moradian-Oldak, a dentistry professor at the Herman Ostrow School of Dentistry of USC, has investigated methods to regrow tooth enamel for the past two decades. The process is especially tricky because unlike bone, mature enamel cannot rejuvenate. Tooth enamel is a nonliving tissue.

The a-ha moment came October 22 when, in collaboration with lead author Sauma Prajapati of USC and other colleagues, she published a study in the Biomaterials journal saying matrix metalloproteinase-20, an enzyme found only in teeth, chops up amelogenin proteins, which facilitate organized enamel crystal formation. MMP-20 clears the way for hard material to usurp vacated space.

Her team is the first to define the function of an enzyme for preventing protein occlusion inside a crystal, she said.

“MMP-20 is released at a very early stage of enamel formation,” said Moradian-Oldak, the study’s senior author. “MMP-20 chops up proteins during the crystallization of enamel. Together with other enzymes, it gets rid of ‘sludge’ so the enamel making cells in the body can add more mineral and make enamel, the hardest bioceramic in the human body.”

Repairing tooth decay
Moradian-Oldak will marry the MMP-20 discovery with another study published Nov. 2 in the Journal of Biomedical Engineering and Informatics, which concluded an amelogenin-chitosan hydrogel could repair early tooth decay by growing an enamel-like layer that reduces lesions by up to 70%.

“Recognizing MMP-20’s function in biomineralization is one of the first steps to learning how dental enamel forms in nature,” said Qichao Ruan, lead author of the hydrogel study and a postdoctoral research associate in the Center for Craniofacial Molecular Biology at USC. “The findings regarding MMP-20 not only help us to further understand the mechanisms of enamel formation but also can be applied in the design of novel biomaterials for future clinical applications in dental restoration or repair.”

The Food and Drug Administration has not yet approved any type of enamel regrowing gel. USC is in preclinical trials. Moradian-Oldak said one day people may be able to use an overnight mouth guard or teeth strips saturated with hydrogel to regrow enamel-like substances and reduce teeth sensitivity.

Finding the right fix
Products such as toothpaste and mouthwash containing fluoride and casein phosphopeptide-amorphous calcium phosphate promote remineralization of initial enamel lesions; however, they need to be used regularly and are more of a tire patch than a real solution, Moradian-Oldak said. It plugs up the problem so people don’t feel pain. The gel, however, fills the cracks and holes with an enamel-like substance.

In the United States, 92% of adults aged 20 to 64 have had dental decay in their permanent teeth, Moradian-Oldak said. Grinding teeth at night, gum recession, and the disappearance of enamel over a lifetime due to demineralizing acidic food and drink are all common problems people everywhere face.

When tested in an environment that mimics an oral cavity’s biochemical processes, the gel created a robust attachment, eliminating the threat of secondary cavities in the same spot, Ruan said. The gel could be more effective than traditional crowns, whose adhesion weakens over time, he added.

“Besides biocompatibility and biodegradability, the gel has unique antimicrobial and adhesion properties that are important for dental applications,” Ruan said.

MMP-20 carves out proteins that decrease enamel strength
USC researchers tested their theory using wild type mice and MMP-20 null mice. The MMP-20 null mice had inconsistent enamel hydroxyapatite crystals that were shorter, wider, and thinner than those found in the wild type mice.

Some 31% of the enamel nanocrystals area isolated from MMP-20 mice were imperfect, whereas only 10% of the area was imperfect in crystals from wild type mice.

The gel that produces enamel-like growth
In preparation for a possible human study, USC researchers used human molars without any lesions. They sliced teeth into three or four blocks, created artificial tooth decay, then cycled the samples in artificial saliva with pH 4.6, 7.0, and 6.5.

Normal salivary pH is between 6 to 7 but could quickly fluctuate between 5.3 to 7.8 based on food and beverage intake.

A sample of supersaturated calcium and phosphate ions in a remineralization solution produced an enamel-like substance; however, it created a disorganized structure with irregular crystals. In contrast, the hydrogel grew oriented crystals, reducing the depth of the lesions by 50% to 70% after seven days of hydrogel application. It is a big improvement over other methods, Ruan said.

“In one study, it was reported that only about 24% of tooth decay was recovered after 12 days of pH cycling with sodium fluoride treatment,” he said.

The next step is to alter the gel recipe using MMP-20 to create a stronger enamel-like seal, Moradian-Oldak said.

“We create a protective cover on enamel,” she said. “We restore the structure of enamel, and it will prevent decay from progressing.”

The studies were funded by grants from the National Institutes of Health’s National Institute of Dental and Craniofacial Research (DE-13414 and DE-020099) and USC Coulter Translational Research Partnership Program via the Wallace Coulter Foundation (WCF/GRZYWACZ/2011).

 

Source: Dentistry IQ

How to Heal Cavities Naturally

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Update: Researchers have possibly found a possibility to regrow teeth. For more information click here

The world is slowly waking up to the fact that, when you give the body what it needs, it can heal things we previously thought were impossible. A fine example of what is often deemed as an incurable health problem is dental cavities, but extensive research is now becoming more public about the true nature of tooth decay and the fact that there are proven remedies that can remedy it.

The lies perpetrated about tooth decay

According to the American Dental Association, the reason we have tooth decay is as follows:

“[Tooth decay] occurs when foods containing carbohydrates (sugars and starches) such as milk, pop, raisins, cakes or candy are frequently left on the teeth. Bacteria that live in the mouth thrive on these foods, producing acids as a result. Over a period of time, these acids destroy tooth enamel, resulting in tooth decay.”

There are a few problems with this theory, including:

  • Groups of indigenous people who had fermentable carbohydrates stuck on their teeth all the time that did not brush or floss were mostly or completely free of tooth decay.
  • Bacteria do not consume processed sugar or flour because of the lack of nutrients in them.
  • Foods that bacteria like to eat, such as milk, vegetables, meat, fish and fruit, are not commonly implicated in causes of tooth decay.

So if the modern explanation of tooth decay is not accurate, what is actually the cause of tooth decay?

What actually causes tooth decay

Tooth decay, as researched by Dr. Weston Price and other dental pioneers, boiled down to three factors:

  • Not enough minerals in the diet.
  • Not enough fat-soluble vitamins (A, D, E, and K) in the diet.
  • Nutrients not being readily bioavailable, and your intestinal system not properly absorbing them.The presence of phytic acid largely influences this factor.

Over a period of time, if your diet lacks vitamins and minerals from a poor diet and/or contains high levels of phytates (from grains, seeds, nuts, and legumes), the blood chemistry and the ratio of calcium and phosphorous become out of balance, which results in minerals being pulled from bones, causing tooth and bone loss.

So, the long-standing belief that sugar causes tooth decay is true, but as a result of it depleting nutrients from the body, not because bacteria eat it and produce acid that ruins your teeth.

The food remedies that can heal cavities and tooth decay

In order to restore the ratio of calcium and phosphorus in our blood, and to enable minerals to bond to our teeth, it is not enough to just avoid eating too many sweet or processed foods. We must also eat health-building foods, containing copious amounts of minerals and vitamins that will build a glassy hard tooth structure.

Foods to focus on are:

  • Coconut oil, grass-fed organic dairy (especially butter), grass-fed meats, seafood and bone broths.
  • Organic cooked vegetables (soups with bone broth are ideal).
  • Organ and gland meats, like liver.

Limit foods that are high in phytic acid, like grains, beans, nuts and seeds, as well as limiting processed food intake full of processed flours and sugars that upset blood sugar balance.

Supplements to consider are:

  • Fermented cod liver oil – very high in fat soluble vitamins A, D and K.
  • Magnesium – required to use calcium and phosphorous effectively.
  • Gelatin – if you don’t have time to make bone broth, this is a good alternative and is great for gums and digestion.

Now go get your pearly white smile back.

See full article here: Healthy Holistic Living